Description:
An enzyme immunoassay for the quantitative measurement of CA15-3 in serum and plasma.
The DRG TM-CA 15-3 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the CA15-3 molecule. An aliquot of patient sample containing endogenous CA15-3 is incubated in the coated well with enzyme conjugate, which is an anti-CA15-3 antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CA15-3 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA15-3 in the patient sample.