This assay is an enzyme immunoassay intended for the quantitative determination of calprotectin (MRP (8/14, S100A8/A9) in serum and plasma. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The assay utilizes the two-site sandwich technique with two selected monoclonal antibodies that bind to human
Calprotectin. Standards, controls and diluted patient samples which are assayed for human calprotectin are added to wells of microplate coated with a high affine monoclonal anti-human Calprotectin antibody. During the first incubation step, Calprotectin in the samples is bound by the immobilized antibody. Then a peroxidase labeled conjugate is added to each well and the following complex is formed: capture antibody - human calprotectin Peroxidase conjugate.
Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the calprotectin concentration of sample. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from standard. Calprotectin, present in the patient samples, is determined directly from this curve.