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Fasciola IgG ELISA RUO

  • Bio-Type Serum/Plasma
  • Method ELISA
  • FDA RUO
  • CE N
  • # of Tests 96 wells
  • Range 0.26 – 60 DU/mL
  • Sample Volume 10 uL
  • Incubation Time(s) 60 / 30 / 15 min
  • Storage Conditions 2° C - 8° C
  • SKU:  EIA4503R
  • Category: Infectious Diseases
  • $470.00
Approximate Lead Time 1 - 2 Weeks
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Description:

An enzyme immunoassay for the qualitative and semiquantitative determination of IgG-class antibodies to Fasciola in serum and plasma (EDTA-, or heparin- plasma). The DRG Fasciola IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with Fasciola antigen from adult liver fluke. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Fasciola-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Fasciola-specific IgG antibody in the patient specimen.  Absorbance at 450 nm is read using an ELISA microtiter plate reader.