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Malaria ELISA RUO

  • Bio-Type Serum/Plasma
  • Method ELISA
  • FDA RUO
  • CE N
  • # of Tests 96 wells
  • Range Qualitative
  • Sensitivity 97.5%
  • Sample Volume 10 uL
  • Incubation Time(s) 60 / 30 / 15 min
  • Storage Conditions 2° C - 8° C
  • SKU:  EIA5511R
  • Category: Infectious Diseases
  • $242.00
Approximate Lead Time 1 - 2 Weeks
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Description:

The Malaria ELISA is intended for the qualitative determination of IgG/IgM antibodies against Plasmodium in human serum or plasma (citrate, heparin). This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.