Rubella IgG ELISA RUO

The DRG Rubella Virus IgG Enzyme Immunoassay Kit provides materials for the quantitative and qualitative determination of IgG-class antibodies to Rubella Virus in human serum and plasma. The DRG Rubella Virus IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with inactivated K1S grade Rubella Virus antigen (strain HPV-77). Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Rubella Virus-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Rubella Virus-specific IgG antibody in the patient specimen.  Absorbance at 450 nm is read using an ELISA microtiter plate reader.