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HSV-1 IgM ELISA RUO

  • Bio-Type Serum/Plasma
  • Method ELISA
  • FDA RUO
  • CE N
  • # of Tests 96 wells
  • Range 0.16 – 60 DU/mL
  • Sample Volume 10 uL
  • Incubation Time(s) 60 / 30 / 15 min
  • Storage Conditions 2° C - 8° C
  • SKU:  EIA3486R
  • Category: Infectious Diseases
  • $150.00
Approximate Lead Time 1 - 2 Weeks
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Description:

The DRG Herpes Simplex Virus Type 1 IgM Enzyme Immunoassay Kit provides materials for the qualitative and semiquantitative determination of IgM-class antibodies to HSV-1 in human serum and plasma. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The DRG Herpes Simplex Virus Type 1 IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Patient samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results. Microtiter wells as a solid phase are coated with recombinant gG1 protein of HSV-1.  Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation HSV-1-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of HSV-1-specific IgM antibody in the patient specimen.  Absorbance at 450 nm is read using an ELISA microtiter plate reader.