Immunoenzymatic colorimetric method for quantitative determination of hNSE concentration in human serum.
The NSE ELISA test is based on simultaneous binding of human Neuron Specific Enolase by two monoclonal antibodies, one immobilized on microwell plates and the other conjugates with horseradish peroxidase (HPR). After incubation the bound/free separation is performed by a simple solid-phase washing, then the TMB-Substrate solution (TMB) is added. After an appropriate time has elapsed for maximum colour development, the enzyme reaction is stopped and the absorbancies are determinated. The colour intensity is proportional to the hNSE concentration in the sample. hNSE concentration in the sample is calculated based on a Calibration curve.