An enzyme immunoassay for the quantitative measurement of TSH in serum or plasma. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The DRG TSH ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the TSH molecule. An aliquot of patient sample containing endogenous TSH is incubated in the coated well with enzyme conjugate, which is an anti-TSH antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of TSH in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of TSH in the patient sample.