An enzyme immunoassay for the quantitative measurement of CA 72-4 (TAG-72) in serum and plasma. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The DRG TM-CA 72-4 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal mouse antibody (Clone CC49) directed towards a unique antigenic site on a CA 72-4 molecule. An aliquot of patient sample containing endogenous CA 72-4 is incubated in the coated well with enzyme conjugate, which is an anti-CA 72-4 antibody (Clone B72.3) conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CA 72-4 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA 72-4 in the patient sample.