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  • Bio-Type Serum/Plasma
  • Method ELISA
  • CE N
  • # of Tests 96 wells
  • Range 0.2 – 240 U/mL
  • Sensitivity 0.2 U/ml
  • Sample Volume 50 uL
  • Incubation Time(s) 60 / 60 / 30 min
  • Storage Conditions 2° C - 8° C
  • SKU:  EIA5069R
  • Category: Tumor Marker
  • $211.00
Approximate Lead Time 1 - 2 Weeks
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An enzyme immunoassay for the quantitative measurement of CA 19-9 in serum or plasma (EDTA or heparin plasma). The DRG TM-CA 19-9 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the CA 19-9 molecule.  An aliquot of patient sample containing endogenous CA 19-9 is incubated in the coated well with assay buffer. After a washing step a second incubation follows with enzyme conjugate, which is an anti-CA 19-9 antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CA 19-9 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA 19-9 in the patient sample.