An enzyme immunoassay for the quantitative measurement of CA 125 in serum and plasma. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The DRG TM-CA 125 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the CA 125 molecule. An aliquot of patient sample containing endogenous CA 125 is incubated in the coated well with enzyme conjugate, which is a monoclonal anti-CA 125 antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is proportional to the concentration of CA 125 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA 125 in the patient sample.