Description:
The Sperm Antibody ELISA from DRG is a reliable and quantitative test for the determination antibodies directed against human spermatozoa. This test is intended for the use with seminal plasma.
Please note: the terms “anti-spermatozoa antibodies”, “anti-sperm antibodies” and “sperm antibodies” are equivalent. In these instructions the rather unwieldy but correct term “anti-spermatozoa antibodies” is used.
The Sperm Antibody ELISA is a part in clinical practice to diagnose immunologically caused infertility in men. Antibodies directed against spermatozoa antigens may cause infertility in women or men. The application of the AntiSpermatozoa Antibody ELISA from DRG is recommended for the diagnosis of immunologically caused disorders of
fertility.
The DRG Sperm Antibody ELISA (Enzyme Linked ImmunoSorbent Assay) is a solid-phase sandwich enzyme-immunoassay for the quantitative determination of anti-spermatozoa antibodies in human seminal plasma. The ELISA-plate is coated with a mix of spermatozoa proteins which are recognized by anti-spermatozoa antibodies. The samples and standards are pipetted into the wells and then incubated. During this incubation anti-spermatozoa antibodies bind to the spermatozoa proteins and are thus immobilised on the plate. After washing the enzyme conjugate, consisting of anti-human globulin antibodies covalently coupled to horseradish peroxidase, is added. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3,3’,5,5’-tetramethylbenzidine) yielding a colour reaction which is stopped with 0.25 M sulphuric acid (H2SO4). The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength ? 550 nm is recommended.