Ecommerce ordering is currently available for USA customers. International customers should contact their sales representative for orders.

Sperm Antibody ELISA

  • Bio-Type Serum
  • Method ELISA
  • FDA IVD
  • CE Y
  • # of Tests 96 wells
  • Range 0 – 250 U/mL
  • Sample Volume 5uL
  • Incubation Time(s) 60 / 60 / 30 min
  • Storage Conditions 2° C - 8° C
  • SKU:  EIA1826
  • Category: Fertility/Pregnancy
  • $272.00
Approximate Lead Time 1 - 2 Weeks
For Bulk Orders of 35+ Kits, please Contact Us

Description:

An enzyme immunoassay for the quantitative determination of antibodies directed against spermatozoa antigens in serum. The Sperm Antibody ELISA (Enzyme Linked ImmunoSorbent Assay) is a solid-phase sandwich enzyme-immunoassay for the quantitative determination of anti-spermatozoa antibodies in human serum. The ELISA-plate is coated with a mix of spermatozoa proteins which are recognized by anti-spermatozoa antibodies. The samples and standards are pipetted into the wells and then incubated. During this incubation anti-spermatozoa antibodies bind to the spermatozoa proteins and are thus immobilised on the plate. After washing the enzyme conjugate, consisting of anti-human globulin antibodies covalently coupled to horseradish peroxidase, is added. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3,3’,5,5’-tetramethylbenzidine) yielding a color reaction which is stopped with 0.25 M sulphuric acid (H2SO4). The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength ?550 nm is recommended.