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Salivary DHEA-S ELISA RUO

  • Bio-Type Saliva
  • Method ELISA
  • FDA RUO
  • CE N
  • # of Tests 96 Wells
  • Range 0.05 – 12 ng/mL
  • Sensitivity 25 pg/mL
  • Sample Volume 50 uL
  • Incubation Time(s) 15 / 15 min
  • Storage Conditions 2° C - 8° C
  • SKU:  SLV4409R
  • Category: Fertility/Pregnancy
  • $225.00
Approximate Lead Time 1 - 2 Weeks
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Description:

Competitive immunoenzymatic colorimetric method for quantitative determination of DHEA-S concentration in saliva. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The DHEA-S (antigen) in the sample competes with the antigenic DHEA-S conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti DHEA-S coated on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue colour that changes into yellow when the Stop Solution (H2SO4) is added. The colour intensity is inversely proportional to the DHEA-S concentration of in the sample. DHEA-S concentration in the sample is calculated through a calibration curve.