The DRG NSE ELISA RUO is an immunoenzymatic colorimetric method for quantitative determination of hNSE concentration in human sera. This product is for Research Use Only. The NSE ELISA is based on simultaneous binding of human Neuron Specific Enolase by two monoclonal antibodies, one immobilized on microwell plates and the other conjugates with horseradish peroxidase(HRP).
After incubation the bound/free separation is performed by a simple solid-phase washing, then the TMB-Substrate solution (TMB) is added. After an appropriate time has elapsed for maximum color development, the enzyme reaction is stopped and the absorbancies are determined. The color intensity is proportional to the hNSE concentration in the sample.