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Salivary IgA ELISA RUO

  • Bio-Type Saliva
  • Method ELISA
  • FDA RUO
  • CE N
  • # of Tests 96 Wells
  • Range 0.5 – 400 ug/mL
  • Sensitivity 0.5 pg/mL
  • Sample Volume 50 uL
  • Incubation Time(s) 60 / 15 min
  • Storage Conditions 2° C - 8° C
  • SKU:  SLV4636R
  • Category: Immunology/Hematology
  • $225.00
Approximate Lead Time 1 - 2 Weeks
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Description:

Immunoenzymatic colorimetric method for quantitative measurement of IgA in saliva. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. Salivary IgA ELISA is based on the simultaneous binding of human IgA to two antibodies, one monoclonal immobilized on microwell plates and the other, polyclonal conjugated with horseradish peroxidase (HRP). After incubation the bound/free separation is performed by a simple solid-phase washing. Then the enzyme in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added. The color intensity is proportional to the IgA concentration in the sample. The IgA concentration in the sample is calculated through a standard curve.