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Helicobacter Pylori IgM ELISA RUO

  • Bio-Type Serum
  • Method ELISA
  • FDA RUO
  • CE N
  • # of Tests 96 wells
  • Range Qualitative
  • Sample Volume 5 uL
  • Incubation Time(s) 30 / 30 / 20 min
  • Storage Conditions 2° C - 8° C
  • SKU:  EIA2111R
  • Category: Infectious Diseases
  • $150.00
Approximate Lead Time 1 - 2 Weeks
For Bulk Orders of 35+ Kits, please Contact Us

Description:

The DRG Helicobacter pylori IgM ELISA RUO is an enzyme immunoassay for the detection of IgM antibodies to Helicobacter pylori in serum.  This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version.

Purified H. pylori antigen is coated on the surface of microwells.  Diluted patient serum is added to the wells, and the H. pylori IgM specific antibody, if present, binds to the antigen.  All unbound materials are washed away.  Enzyme conjugate is added, which binds to the antibody-antigen complex.  Excess enzyme conjugate is washed off and a solution of TMB Reagent is added.  The enzyme conjugate catalytic reaction is stopped at a specific time.  The intensity of the color generated is proportional to the amount of IgM-specific antibody in the sample.  The results are read by a microwell reader compared in a parallel manner with calibrator and controls.