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Anti ß2-Glycoprotein 1 IgG/IgM ELISA

  • Bio-Type Serum/Plasma
  • Method ELISA
  • FDA No
  • CE Y
  • # of Tests 96 wells
  • Range 0 – 100 U/mL (IgG and IgM)
  • Sample Volume 10 uL
  • Incubation Time(s) 30 / 15 / 15 / 5 min
  • Storage Conditions 2° C - 8° C
  • SKU:  EIA3590
  • Category: Autoimmune Diseases
Approximate Lead Time 1 - 2 Weeks
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Description:

Anti-beta-2-Glycoprotein I IgG/IgM is an ELISA test system for the quantitative measurement of IgG and IgM class autoantibodies against beta-2-Glycoprotein I in human serum or plasma. Highly purified beta-2-Glycoprotein I is bound to microwells. The determination is based on an indirect enzyme linked immune reaction with the following steps: Specific antibodies in the patient sample bind to the antigen coated on the surface of the reaction wells. After incubation, a washing step removes unbound and unspecifically bound serum or plasma components. Subsequently added enzyme conjugate binds to the immobilized antibody-antigen-complexes. After incubation, a second washing step removes unbound enzyme conjugate. After addition of substrate solution the bound enzyme conjugate hydrolyses the substrate forming a blue coloured product. Addition of an acid stops the reaction generating a yellow end-product. The intensity of the yellow color correlates with the concentration of the antibody-antigen-complex and can be measured photometrically at 450 nm.