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Free ß-HCG ELISA RUO

  • Bio-Type Serum/Plasma
  • Method ELISA
  • FDA RUO
  • CE N
  • # of Tests 96 wells
  • Range 0.2 – 200 ng/mL
  • Sensitivity 0.2 ng/ml
  • Sample Volume 50 uL
  • Incubation Time(s) 30 / 30 / 20 min
  • Storage Conditions 2° C - 8° C
  • SKU:  EIA4718R
  • Category: Fertility/Pregnancy
  • $200.00
Approximate Lead Time 1 - 2 Weeks
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Description:

An enzyme immunoassay for the quantitative measurement of free beta subunit of human chorionic gonadotropin (free Beta-hCG) in serum and EDTA plasma. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The DRG Free Beta-HCG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site on a Free Beta-hCG molecule.  An aliquot of patient sample containing endogenous Free Beta-hCG is incubated in the coated well with enzyme conjugate, which is an anti-Beta-HCG antibody [rabbit] conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of Free Beta-HCG in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of Free Beta-HCG in the patient sample.