Ecommerce ordering is currently available for USA customers. International customers should contact their sales representative for orders.
Disclaimer: The kit labels displayed in each photo on this website do not necessarily represent the regulatory status of each product for every country of our customers.

IL-6 ELISA

  • Bio-Type Serum
  • Method ELISA
  • FDA No
  • CE Y
  • # of Tests 96 wells
  • Range 2 -2560 pg/mL
  • Sensitivity 2 pg/mL
  • Sample Volume 100 ulL
  • Incubation Time(s) 60 / 15 min
  • Storage Conditions 2° C - 8° C
  • SKU:  EIA4640
  • $834.00
Approximate Lead Time 1 - 2 Weeks
For Bulk Orders of 35+ Kits, please Contact Us

Description:

The DRG IL 6-ELISA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplate. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of IL 6. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 – human IL 6 – MAb 2 – HRP, the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic reaction.

Chromogenic solution (TMB) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is proportional to the IL 6 concentration.

A calibration curve is plotted and IL 6 concentration in samples is determined by interpolation from the calibration curve.