An enzyme immunoassay for the quantitative measurement of aldosterone in serum, plasma (EDTA-, heparin- or citrate plasma) and urine. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The DRG Aldosterone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal rabbit antibody directed towards an antigenic site of the aldosterone molecule. Endogenous aldosterone of a patient sample competes with an aldosterone-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of aldosterone in the patient sample.