An enzyme immunoassay for the quantitative measurement of alpha fetoprotein (AFP) in serum. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The DRG AFP ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site on an AFP molecule. An aliquot of sample containing endogenous AFP is incubated in the coated well with enzyme conjugate, which is an anti- AFP antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of AFP in the sample. Having added the substrate solution, the intensity of color developed is proportional to the concentration of AFP in the sample.