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  • Bio-Type Serum/Plasma
  • Method ELISA
  • CE Y
  • # of Tests 96 Wells
  • Range 0.35 - 1166 pg/mL
  • Sensitivity 0.35 pg/mL
  • Sample Volume 200 uL
  • Incubation Time(s) 120 / 15 min
  • Storage Conditions 2° C - 8° C
  • SKU:  EIA4437
  • $834.00
Approximate Lead Time 1 - 2 Weeks
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The IL-1β ELISA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiter plate. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of IL-1β. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 – human IL-1β – MAb 2 – HRP, the microtiter plate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic reaction.

Chromogenic solution (TMB) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiter plate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is proportional to the IL-1β concentration.

A calibration curve is plotted and IL-1β concentration in samples is determined by interpolation from the calibration curve.