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Troponin I ELISA RUO

  • Bio-Type Serum
  • Method ELISA
  • CE N
  • # of Tests 96 wells
  • Range 0.75 – 75 ng/mL
  • Sample Volume 100 uL
  • Incubation Time(s) 90 / 20 min
  • Storage Conditions 2° C - 8° C
  • SKU:  EIA2952R
  • Category: Cardiovascular/Hypertension
  • $300.00
Approximate Lead Time 1 - 2 Weeks
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An enzyme immunoassay for the quantitative determination of caridac specific troponin I in serum. This test is intended for the use with serum. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The cTnI ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay.  The assay system utilizes four unique monoclonal antibodies directed against distinct antigenic determinants on the molecule.  Three mouse monoclonal anti-troponin I antibodies are used for solid phase immobilization (on the microtiter wells). The fourth antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution.  The test sample is allowed to react simultaneously with the four antibodies, resulting in the troponin I molecules being sandwiched between the solid phase and enzyme-linked antibodies.  After a 90-minute incubation at room temperature, the wells are washed with water to remove unbound-labeled antibodies.  A solution of tetramethylbenzidine (TMB) Reagent is added and incubated for 20 minutes, resulting in the development of a blue color.  The color development is stopped with the addition of 1N hydrochloric acid (HCl) changing the color to yellow.   The concentration of troponin I is directly proportional to the color intensity of the test sample.  Absorbance is measured spectrophotometrically at 450 nm.