The enzyme-linked immunosorbent assay (ELISA) is intended for the quantitative or qualitative determination of IgA antibodies in human serum, directed against gliadin. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The immobilized antigen is a gliadin preparation purified from wheat gluten. The test is fast (incubation time 30 / 30 / 30 minutes) and flexible (divisible solid phase, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control check the assay performance.
The wells of the solid phase are coated with gliadin. On this surface, the following immunological reactions take place:
1st reaction: gliadin-specific antibodies present in the sample bind to the immobilized antigen, forming the antigen-antibody complex. Then, non-bound sample components are washed away from the solid phase.
2nd reaction: A second antibody, directed at human IgA antibodies and conjugated with horse-radish peroxidase (HRP), is added. This conjugate binds to the complex. Then, excess conjugate is washed away from the solid phase.
3rd reaction: The enzyme-labelled complex converts a colorless substrate into a blue product. The degree of color development reflects the concentration of gliadin antibodies (IgA) in the sample.