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Gliadin Ab IgA ELISA RUO

  • Bio-Type Serum
  • Method ELISA
  • FDA RUO
  • CE N
  • # of Tests 96 wells
  • Range 1 – 100 U/mL
  • Sample Volume 100 uL
  • Incubation Time(s) 30 / 30 / 30 min
  • Storage Conditions 2° C - 8° C
  • SKU:  EIA4069R
  • Category: Allergy Testing
  • $190.00
Approximate Lead Time 1 - 2 Weeks
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Description:

The enzyme-linked immunosorbent assay (ELISA) is intended for the quantitative or qualitative determination of IgA antibodies in human serum, directed against gliadin. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The immobilized antigen is a gliadin preparation purified from wheat gluten. The test is fast (incubation time 30 / 30 / 30 minutes) and flexible (divisible solid phase, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control check the assay performance. The wells of the solid phase are coated with gliadin. On this surface, the following immunological reactions take place: 1st reaction: gliadin-specific antibodies present in the sample bind to the immobilized antigen, forming the antigen-antibody complex. Then, non-bound sample components are washed away from the solid phase. 2nd reaction: A second antibody, directed at human IgA antibodies and conjugated with horse-radish peroxidase (HRP), is added. This conjugate binds to the complex. Then, excess conjugate is washed away from the solid phase. 3rd reaction: The enzyme-labelled complex converts a colorless substrate into a blue product. The degree of color development reflects the concentration of gliadin antibodies (IgA) in the sample.